Next, shave the medial aspect of the right hind limb of the mouse to expose the surgical area and disinfect with iodine plus 70%alcohol skin scrub over three alternating cycles. Lastly, allow four weeks of recovery time to ensure shared circulation between parabionts if performing tibial fracture surgery. House each pair individually and be sure to monitor the pairs daily by performing body condition scoring twice weekly and weighing the pairs twice weekly. Complete interrupted sutures with mouse in prone position.Īllow the mice to awaken in ambient air. Next, join the quadriceps of the animal with two interrupted sutures and close the skin of the two parabionts with interrupted sutures. Then join the body walls along the flanks with a running continuous suture of seven to nine passes. Join the triceps of the animals with two interrupted sutures. On each mouse, use a scalpel to make a skin incision along the flank ranging proximal to the knee to just proximal to the elbow and without disturbing the muscles underneath the skin. Inject 0.25%bupivacaine at the flanks just before opening. Begin by administering analgesia after induction and before surgical manipulation. Disinfect with iodine plus 70%alcohol skin scrub over three alternating cycles in preparation for incision. Next, shave each of the two mice along a continuous line from the elbow, the flank, and the knee on the side to be joined. Finally, maintain correct level of anesthesia for each mouse. After administering general anesthesia, place the mice on a heated pad in the supine position. To prepare for surgery, weigh the two mice. ![]() Check the body condition and overall appearance of mice daily. For parabiosis, acclimate two mice together for at least two weeks before surgery. Use one female mouse at three months of age and one old mouse at 17 months of age. House no more than five litter mates per cage and avoid conditions that could encourage fighting. Prior to the experiment, keep mice in an air conditioned environment with 12-hour light and dark cycles and proper access to standard food and water. The main advantage of the orthopedic procedure is the clinical applicability of these findings to common complications following surgery, for example delirium and post-operative cognitive dysfunction. These methods can help answer key questions related to the field of immunology, aging, tissue regeneration, and cognitive function. One of the mice within this model will then be fractured to observe the regenerative effects and the effect on cognitive function in relation to the shared system of the young and old mouse. ![]() The overall goal of these surgical methods is to observe the effects of trauma on multi-organ dysfunction, specifically parabiosis will be used to anastomosis of a young and old mouse together resulting in a shared blood supply.
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